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Candida albicans promotes invasion and colonisation of Candida glabrata in a reconstituted human vaginal epithelium

Alves, Carlos Tiago, Wei, Xiao-Qing, Silva, Sónia, Azeredo, Joana, Henriques, Mariana and Williams, David Wynne 2014. Candida albicans promotes invasion and colonisation of Candida glabrata in a reconstituted human vaginal epithelium. Journal of Infection 69 (4) , pp. 396-407. 10.1016/j.jinf.2014.06.002

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Abstract

Objective The principal aim of this study was to investigate the in vitro co-infection of a reconstituted human vaginal epithelium (RHVE) by Candida albicans and Candida glabrata. Methods The ability of both species to invade and colonise the RHVE was examined using species-specific peptide nucleic acid (PNA) probe hybridisation, confocal laser scanning microscopy (CLSM) and a novel qRT-PCR protocol for Candida quantification in the tissues. RHVE damage was evaluated by measuring lactate dehydrogenase (LDH) activity. Candida virulence gene expression (HWP1, ALS, EPA, PLB, PLD and SAP) was evaluated by quantitative RT-PCR. Results The results showed that whilst both species induced damage to the RHVE, this was notably less with C. glabrata. Interestingly, there was a significant increase in C. glabrata RHVE colonisation and invasiveness when it was added to the tissue with C. albicans. The extent of RHVE damage caused by the two species appeared to be primarily dependent on the process of invasion. Of the virulence genes assayed, HWP1, PLD1 and ALS3 were deemed to be most associated with pathogenicity in the model. Conclusions For the first time, we have demonstrated that the RHVE model coupled with specific tools of analysis, allows assessment of Candida colonisation and invasion in single and co-infection. Using this model we have demonstrated that C. albicans enhanced C. glabrata colonisation, invasion and tissue damage, which was also evidenced by the expression of virulence genes.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: Q Science > QR Microbiology
Uncontrolled Keywords: RHVE; candidosis; co-infection; candida albicans; candida glabrata; yeast PNA FISH
Publisher: Elsevier
ISSN: 0163-4453
Date of Acceptance: 2 June 2014
Last Modified: 28 Feb 2019 14:49
URI: http://orca-mwe.cf.ac.uk/id/eprint/60614

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