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Crystallization and preliminary crystallographic analysis of the fusion core from two new zoonotic paramyxoviruses, Nipah virus and Hendra virus

Xu, Yanhui, Lou, Zhiyong, Liu, Yiwei, Cole, David, Su, Nan, Qin, Lan, Li, Xu, Bai, Zhihong, Rao, Zihe and Gao, George F. 2004. Crystallization and preliminary crystallographic analysis of the fusion core from two new zoonotic paramyxoviruses, Nipah virus and Hendra virus. Acta Crystallographica Section D: Biological Crystallography 60 (6) , pp. 1161-1164. 10.1107/S0907444904009515

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Abstract

Highly conserved heptad-repeat (HR1 and HR2) regions in class I viral fusion (F) proteins, including the F protein from paramyxovirus, interact with each other post-fusion to form a six-helix bundle called a fusion core. Crystals of the fusion core of Nipah virus have been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.1 Å resolution at 100 K in-house. The crystals have unit-cell parameters a = 31.664, b = 31.725, c = 51.256 Å, α = 80.706, β = 86.343, γ = 65.812° and belong to space group P1. Crystals of the fusion core of Hendra virus have also been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.0 Å resolution at 100 K in-house. A selenomethionine (SeMet) derivative of the HeV fusion core was overexpressed using the same Escherichia coli expression system and purified. The derivative crystals were obtained under similar conditions and three different wavelength data sets were collected to 2.0 Å resolution from the derivative crystal at BSRF (Beijing Synchrotron Radiation Facility). The crystals have unit-cell parameters a = 31.997, b = 31.970, c = 53.865 Å, α = 85.990, β = 85.842, γ = 68.245° and belong to space group P1.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: fusion cores; Nipah virus; Hendra virus
Publisher: International Union of Crystallography
ISSN: 0907-4449
Last Modified: 04 Jun 2017 06:28
URI: http://orca-mwe.cf.ac.uk/id/eprint/60528

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