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Promoter polymorphisms in glutathione-S-transferase genes affect transcription

Guy, Carol, Hoogendoorn, Bastiaan, Smith, Susan Kay, Coleman, Sharon Louise, O'Donovan, Michael Conlon and Buckland, Paul Robert 2004. Promoter polymorphisms in glutathione-S-transferase genes affect transcription. Pharmacogenetics 14 (1) , pp. 45-51. 10.1097/00008571-200401000-00005

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Abstract

The glutathione-S-transferases are a group of enzymes that play a major role in detoxification and defence against toxic, carcinogenic and other compounds. We analysed the proximal promoters of 14 genes encoding glutathione-S-transferase for polymorphism. Ten of the promoters contained sequence variants, nine of which we were able to clone into a reporter gene vector, pGL3. The relative ability of each haplotype to promote transcription of the luciferase gene was tested in each of two human cell lines (HEK293t and TE671) using a cotransfected CMV-SEAP plasmid as a control. Four genes (GSTA1, GSTA2, GSTM4 and GSTT2) showed activity differences greater than 1.5-fold between haplotypes, and a fifth gene (MGST1) showed a 1.4-fold difference. The promoter sequence variants in these genes may therefore play a role in human variation, susceptibility to diseases and the effects of drugs.

Item Type: Article
Date Type: Publication
Status: Published
Schools: MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Medicine
Subjects: R Medicine > R Medicine (General)
ISSN: 0960-314X
Last Modified: 14 Feb 2020 13:11
URI: http://orca-mwe.cf.ac.uk/id/eprint/57987

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