A structural comparison of guinea pig thyroid and fat TSH receptors by photoaffinity labelling

Buckland, Paul Robert and Rees Smith, Bernard 1984. A structural comparison of guinea pig thyroid and fat TSH receptors by photoaffinity labelling. FEBS Letters 166 (1) , pp. 109-114. 10.1016/0014-5793(84)80054-X

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Abstract

TSH receptors from guinea pig thyroid and epididymal fat have been covalently crosslinked to 125I-labelled TSH conjugated to N-hydroxysuccinimidyl-4-azidobenzoate. Analysis by SDS—PAGE and autoradiography showed bands corresponding to TSH subunits (Mr 14 000) and intact TSH (Mr 28 000; subunits crosslinked) and two at higher Mr separated by 14 000. The latter bands represented one or two subunits of TSH crosslinked to a subunit of the TSH receptor with Mr 57 000 (fat) or 60 000 (thyroid). These Mr values were reduced by trypsin treatment to 43 000 and 50 000, respectively. Analysis under non-reducing conditions showed that both fat and thyroid receptors have a second disulphide linked subunit of Mr 30 000

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG) Medicine
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	TSH receptor; Photoaffinity labelling; TSH—TSH receptor complex; Disulphide-linked subunit; Partial trypsin digestion; Guinea pig adipocyte
Publisher:	Elsevier
ISSN:	0014-5793
Last Modified:	05 Feb 2020 03:36
URI:	https://orca.cardiff.ac.uk/id/eprint/57610

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