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Mosaic expression of membrane peptidases by confluent cultures of Caco-2 cells

Howell, Steven, Brewis, Ian Andrew, Hooper, Nigel M., Kenny, A. John and Turner, Anthony J. 1993. Mosaic expression of membrane peptidases by confluent cultures of Caco-2 cells. FEBS Letters 317 (1-2) , pp. 109-112. 10.1016/0014-5793(93)81502-Q

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Abstract

The cell-surface expression of endopeptidase-24.11 (EC 3.4.24.11) on Caco-2 cells cultured to confluency is markedly heterogeneous unlike that of dipeptidylpeptidase IV (EC 3.4.14.5). Here we have investigated the cell-surface expression of three other ectopeptidases: angiotensin converting enzyme (EC 3.4.15.1), aminopeptidase N (EC 3.4.11.2) and aminopeptidase W (EC 3.4.11.16). We show by indirect immunofluorescent staining that these three enzymes are present on the surface of some cells but not on others. However, these enzymes were detected in the majority of detergent-permeabilised Caco-2 cells indicating the presence of intracellular pools of these enzymes. This suggests that there may either be differential regulation of apical transport for these peptidases or that they recycle at different rates.

Item Type: Article
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Elsevier
ISSN: 0014-5793
Last Modified: 04 Jun 2017 06:13
URI: http://orca-mwe.cf.ac.uk/id/eprint/57438

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