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Preservation and transportation of hepatocyte monolayers in a no spill format at non freezing temperatures

Evans, Peter J. 2014. Preservation and transportation of hepatocyte monolayers in a no spill format at non freezing temperatures. International Journal of Biology 6 (2) , pp. 58-63. 10.5539/ijb.v6n2p58

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Abstract

Hepatocyte monolayers were preserved under a gelatin overlay at 10°C. Morphological changes were observed after 4 days, as structure less areas at the cell periphery. The changes could be prevented by adding sucrose (0.05M) to the gel. Cell viability remained high for 7 days. Cell spreading occurred rapidly when the cells were reactivated. Basal enzyme levels were maintained during the preservation period. The hormonal induction of tyrosine aminotransferase was stable for 4 days but declined subsequently. Sucrose addition extended the period of preservation where induction was evident. The method provides a robust system that allows preservation in the absence of potentially deleterious chemicals. Time is allowed to send cells anywhere in the World in a no spill format whist arriving at their destination as if freshly produced.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Publisher: Canadian Center of Science and Education
ISSN: 1916-9671
Date of First Compliant Deposit: 18 February 2019
Date of Acceptance: 14 January 2014
Last Modified: 28 Jun 2019 02:01
URI: http://orca-mwe.cf.ac.uk/id/eprint/57359

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