Analysis of the BglI restriction fragment length polymorphism in the human factor VIII gene using “virtual PCR”– a novel approach employing the polymerase chain reaction in the absence of sequence information for the locus

Bowen, Derrick John and Hampton, K. K. 1996. Analysis of the BglI restriction fragment length polymorphism in the human factor VIII gene using “virtual PCR”– a novel approach employing the polymerase chain reaction in the absence of sequence information for the locus. Human Genetics 98 (2) , pp. 219-222. 10.1007/s004390050195

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Abstract

The BglI restriction fragment length polymorphism (RFLP) of the human factor VIII (FVIII) gene is potentially useful in linkage studies in haemophilia A. The sequence at the RFLP locus is not known, therefore it is not amenable to analysis by the polymerase chain reaction (PCR) and Southern blotting is required. We present a novel approach for analysis of the BglI RFLP using the PCR targeted to known sequence downstream in exon 26 of the FVIII gene. Briefly, the size of the genomic restriction fragment carrying the PCR target depends upon whether the RFLP site is present or absent. If fragments of the required size are isolated from a genomic digest and used as substrates in the exon 26 PCR, the generation of a product in one or other fraction indicates the upstream RFLP status. We have called this approach “virtual PCR”, since PCR is used to obtain information about the RFLP without amplifying the locus itself.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Springer-Verlag
ISSN:	0340-6717
Last Modified:	04 Jan 2023 02:12
URI:	https://orca.cardiff.ac.uk/id/eprint/57207

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