Inhibition of RPE lysosomal and antioxidant activity by the age pigment lipofuscin

Shamsi, Farrukh Anis and Boulton, Michael Edwin 2001. Inhibition of RPE lysosomal and antioxidant activity by the age pigment lipofuscin. Investigative Ophthalmology and Visual Science 42 (12) , pp. 3041-3046.

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Abstract

PURPOSE. To determine whether lipofuscin is detrimental to lysosomal and antioxidant function in cultured human retinal pigment epithelial (RPE) cells. METHODS. Isolated lipofuscin granules were fed to confluent RPE cultures and the cells maintained in basal medium for 7 days. Parallel cultures were established that did not receive lipofuscin. Cultures were either exposed to visible light (390–550 nm) at an irradiance of 2.8 mW/cm2 or maintained in the dark at 37°C for up to 24 hours. Cells were subsequently assessed for cell viability, lysosomal enzyme activity, and antioxidant capacity. RESULTS. There was no loss of cell viability during the first 3 hours of light exposure, whereas a 10% loss of viability was observed in lipofuscin-fed cultures after 6 hours’ exposure to light. Activities of acid phosphatase, N-acetyl-ß-glucuronidase, and cathepsin D were decreased by up to 50% in lipofuscin-fed cells exposed to light compared with either unfed cells or cells maintained in the dark. There was also a decrease in the antioxidant potential of RPE cells. Catalase and superoxide dismutase activities decreased by up to 60% and glutathione levels by 28% in light-exposed lipofuscin-fed cells compared with unfed cells or cells maintained in the dark. CONCLUSIONS. Lipofuscin has the capacity to reduce the efficacy of the lysosomal and antioxidant systems in RPE cells that may play an important role in retinal ageing and the development of age-related macular degeneration.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences
Subjects:	R Medicine > RE Ophthalmology
Additional Information:	Confirmation received by publisher on 21 February 2014 that publisher's pdf can be self-archived 6 months after publication.
Publisher:	Association for Research in Vision and Ophthalmology
ISSN:	0146-0404
Date of First Compliant Deposit:	30 March 2016
Last Modified:	11 May 2023 22:37
URI:	https://orca.cardiff.ac.uk/id/eprint/5091

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