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Utrophin mRNA expression in muscle is not restricted to the neuromuscular junction

Vater, Ruth, Young, Carol, Anderson, Louise V. B., Lindsay, Susan, Blake, Derek J., Davies, Kay E., Zuellig, Richard and Slater, Clarke R. 1998. Utrophin mRNA expression in muscle is not restricted to the neuromuscular junction. Molecular and Cellular Neuroscience 10 (5-6) , pp. 229-242. 10.1006/mcne.1998.0661

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Abstract

Utrophin is normally present exclusively in synaptic regions of skeletal muscle fibers, although it is expressed extrasynaptically in certain pathological situations, where it has been proposed to compensate for the absence of dystrophin in Duchenne muscular dystrophy patients andmdxmice. Recently there have been conflicting reports regarding the preferential expression of utrophin mRNA at the neuromuscular junction. Usingin situhybridization with RNA probes, we show a clear accumulation of autoradiographic labeling at more than 90% of neuromuscular junctions (identified by histochemical demonstration of cholinesterase activity). The intensity of this labeling is proportional to the number of junctional myonuclei in the section. Some clusters of labeling were found associated with nonmuscle nuclei (e.g., blood vessels, nerves), where utrophin is present. In addition, labeling for utrophin mRNA was associated with about 25% of extrajunctional myonuclei, where the protein is not present. The mean labeling per nucleus at junctional myonuclei was at least 10 times greater than at extrajunctional myonuclei. We discuss the possible regulatory mechanisms involved in the heterogeneous expression of utrophin mRNA in skeletal muscle.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Subjects: R Medicine > R Medicine (General)
Publisher: Elsevier
ISSN: 1044-7431
Last Modified: 04 Jun 2017 05:11
URI: http://orca-mwe.cf.ac.uk/id/eprint/49420

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