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Role of β-dystrobrevin in nonmuscle dystrophin-associated protein complex-like complexes in kidney and liver

Loh, Nellie Y., Nebenius-Oosthuizen, Daniela, Blake, Derek J., Smith, Andrew J. H. and Davies, Kay E. 2001. Role of β-dystrobrevin in nonmuscle dystrophin-associated protein complex-like complexes in kidney and liver. Molecular and Cellular Biology 21 (21) , pp. 7442-7448. 10.1128/MCB.21.21.7442-7448.2001

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Abstract

β-Dystrobrevin is a dystrophin-related and -associated protein that is highly expressed in brain, kidney, and liver. Recent studies with the kidneys of the mdx3Cv mouse, which lacks all dystrophin isoforms, suggest that β-dystrobrevin, and not the dystrophin isoforms, may be the key component in the assembly of complexes similar to the muscle dystrophin-associated protein complexes (DPC) in nonmuscle tissues. To understand the role of β-dystrobrevin in the function of nonmuscle tissues, we generated β-dystrobrevin-deficient (dtnb −/−) mice by gene targeting. dtnb −/− mice are healthy, fertile, and normal in appearance. No β-dystrobrevin was detected in these mice by Western blotting or immunocytochemistry. In addition, the levels of several β-dystrobrevin-interacting proteins, namely Dp71 isoforms and the syntrophins, were greatly reduced from the basal membranes of kidney tubules and liver sinusoids and on Western blots of crude kidney and liver microsomes of β-dystrobrevin-deficient mice. However, no abnormality was detected in the ultrastructure of membranes of kidney and liver cells or in the renal function of these mice. β-Dystrobrevin may therefore be an anchor or scaffold for Dp71 and syntrophin isoforms, as well as other associating proteins at the basal membranes of kidney and liver, but is not necessary for the normal function of these mice.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Subjects: R Medicine > R Medicine (General)
Additional Information: Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0270-7306/ (accessed 25/02/2014)
Publisher: American Society for Microbiology
ISSN: 0270-7306
Date of First Compliant Deposit: 30 March 2016
Last Modified: 04 Jun 2017 05:10
URI: http://orca-mwe.cf.ac.uk/id/eprint/49224

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