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Hydroxyl radical modification of human serum albumin generated cross reactive antibodies

Rasheed, Zafar, Khan, Mohd Wajid A. and Ali, Rashid 2006. Hydroxyl radical modification of human serum albumin generated cross reactive antibodies. Autoimmunity 39 (6) , pp. 479-488. 10.1080/08916930600918472

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Abstract

Hydroxyl radical-mediated in vitro modification of human serum albumin (HSA) showed 59.2% hyperchromicity at λmax, 30% loss of alpha helical structure and 71.4% loss of tryptophan fluorescence. The reactive oxygen species (ROS)-modified HSA was highly immunogenic in rabbits as compare to native HSA. The antibody binding was inhibited to the extent of 97% with the immunogen as inhibitor, indicating the induction of immunogen specific antibodies. Experimentally induced antibodies against modified HSA exhibited diverse antigen binding characteristics. Native plasmid DNA, ROS-modified plasmid DNA and ROS-chromatin were found to be an effective inhibitor of induced antibody–immunogen interaction. Induced antibodies against native HSA showed negligible binding to the above mentioned nucleic acid antigens. Band shift assay reiterated the recognition towards nucleic acid antigens. Thus, the induced antibodies against √OH modified HSA resembled the diverse antigen-binding characteristics of naturally occurring systemic lupus erythematosus (SLE) anti-DNA autoantibodies.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Pharmacy
Subjects: Q Science > QR Microbiology
R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords: HSA, oxygen free radicals, ROS–HSA, √OH radical, cross-reactive-antibodies, lupus
Publisher: Informa Healthcare
ISSN: 0891-6934
Last Modified: 18 Dec 2017 11:20
URI: http://orca-mwe.cf.ac.uk/id/eprint/47484

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