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TFIIB recognition elements control the TFIIA-NC2 axis in transcriptional regulation

Deng, Wensheng, Malecova, Barbora, Oelgeschlager, Thomas and Roberts, Stefan G. E. 2009. TFIIB recognition elements control the TFIIA-NC2 axis in transcriptional regulation. Molecular and Cellular Biology 29 (6) , pp. 1389-1400. 10.1128/MCB.01346-08

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TFIIB recognizes DNA sequence-specific motifs that can flank the TATA elements of the promoters of protein-encoding genes. The TFIIB recognition elements (BREu and BREd) can have positive or negative effects on transcription in a promoter context-dependent manner. Here we show that the BREs direct the selective recruitment of TFIIA and NC2 to the promoter. We find that TFIIA preferentially associates with BRE-containing promoters while NC2 is recruited to promoters that lack consensus BREs. The functional relevance of the BRE-dependent recruitment of TFIIA and NC2 was determined by small interfering RNA-mediated knockdown of TFIIA and NC2, both of which elicited BRE-dependent effects on transcription. Our results confirm the established functional reciprocity of TFIIA and NC2. However, our findings show that TFIIA assembly at BRE-containing promoters results in reduced transcriptional activity, while NC2 acts as a positive factor at promoters that lack functional BREs. Taken together, our results provide a basis for the selective recruitment of TFIIA and NC2 to the promoter and give new insights into the functional relationship between core promoter elements and general transcription factor activity.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QR Microbiology
Additional Information: Pdf uploaded in accordance with publisher's policy at (accessed 25/02/2014)
Publisher: American Society for Microbiology
ISSN: 0270-7306
Date of First Compliant Deposit: 30 March 2016
Last Modified: 19 Mar 2016 23:17

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