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COM-1/p8 acts as a tumour growth enhancer in colorectal cancer cell lines

Li, Xin, Martin, Tracey Amanda and Jiang, Wen Guo 2012. COM-1/p8 acts as a tumour growth enhancer in colorectal cancer cell lines. Anticancer Research 32 (4) , pp. 1229-1237.

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Abstract

Background: Candidate of metastasis-1 (COM-1) is a molecule which is stress-induced and cell growth-related. The current study investigated the impact of COM-1 and role of Peroxisome Proliferating Activator Receptor-gamma (PPARγ) agonists on COM-1 knock down in colorectal cancer cells. Materials and Methods: Expression of COM-1 was knocked out in the colorectal cancer cell lines RKO and CaCO2 by transfection with ribozyme transgenes which specifically targeted COM-1. Cell growth, cell migration and apoptosis were measured in wild-type, control and COM-1 knock down cells. The impact or treatment with the PPARγ agonists on the function of COM-1 knock down cells was monitored. Results: COM-1 knock-down cells grew slower than wild-type and control cells. When treated with the PPARγ agonist ciglitazone and bisphenol A diglycidyl ether (BADGE) at several different concentrations, all types of cells grew slower to some extent. Cells with COM-1 knock-down had the same mobility as wild-type and control cells. More apoptotic and dead cells were detected when cells were treated with ciglitazone at 3 μM, especially of COM-1 knock down cells. Conclusion: COM-1 is an anti-apoptotic gene and a cell growth promoter. Furthermore, the PPARγ agonist could increase the inhibitory effect seen in COM-1 knock-down cell growth and to promote apoptosis. In regards to metastasis, COM-1 appears to play no role in cell motility.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords: COM-1, p8, NUPR1, colorectal cancer, cell growth, cell migration, apoptosis, PPARγ agonist
Publisher: The International Institute of Anticancer Research
ISSN: 1791-7530
Last Modified: 04 Jun 2017 04:47
URI: http://orca-mwe.cf.ac.uk/id/eprint/44673

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