Cell envelope proteins of Staphylococcus epidermidis grown in vivo in a peritoneal chamber implant

Modun, B., Williams, P., Pike, W. J., Cockayne, A., Arbuthnott, J. P., Finch, R. and Denyer, Stephen Paul 1992. Cell envelope proteins of Staphylococcus epidermidis grown in vivo in a peritoneal chamber implant. Infection and Immunity 60 (6) , pp. 2551-2553.

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Abstract

Staphylococcus epidermidis was grown in vivo in chambers implanted intraperitoneally in rats. The cell wall and cytoplasmic membrane protein profiles of the in vivo-grown organisms were compared with those of S. epidermidis grown in vitro in nutrient broth (NB), in iron-restricted NB, or in pooled human peritoneal dialysate (HPD). Compared with growth in broth and in common with growth in HPD, growth in vivo in chambers resulted in the repression of many S. epidermidis wall proteins, with proteins of 27, 42, 54, and 70 kDa predominating. Growth in vivo also resulted in the induction of two iron-repressible cytoplasmic membrane proteins of 32 and 36 kDa, which were also present in staphylococci grown in HPD and in iron-restricted NB. Immunoblotting experiments revealed that in sera taken 21 days after inoculation of the intraperitoneal chambers, the predominant antibody response to cell envelope proteins was directed against the 32- and 36-kDa iron-repressible membrane proteins.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Additional Information:	Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0019-9567/ (accessed 24/02/2014)
Publisher:	American Society for Microbiology
ISSN:	0019-9567
Date of First Compliant Deposit:	30 March 2016
Last Modified:	23 May 2023 23:45
URI:	https://orca.cardiff.ac.uk/id/eprint/44249

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