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Study of Staphylococcus aureus collected at slaughter from dairy cows with chronic mastitis

Piccinini, Renata, Tassi, Riccardo, Daprà, Valentina, Pilla, Rachel, Fenner, Jackie, Carter, Ben Richard and Anjum, Muna F. 2012. Study of Staphylococcus aureus collected at slaughter from dairy cows with chronic mastitis. Journal of Dairy Research 79 (2) , pp. 249-255. 10.1017/S002202991200009X

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Abstract

Staphylococcus aureus is one of the most important pathogens associated with bovine mastitis. Recent studies have shown that Staph. aureus strains may differ in virulence, and in their ability to disseminate across commercial dairy herds. The goal of this study was to determine whether Staph. aureus isolates differed in their ability to colonize mammary tissue, and whether such differences could be related to molecular characteristics. Quarter milk and mammary tissues of 22 cows from two dairy herds, were collected at slaughter and bacteriological analysis was performed. All Staph. aureus isolates were characterized by Pulsed Field Gel Electrophoresis (PFGE) and microarray. Overall 45 mammary quarters were infected and 20 Staph. aureus isolates were identified. The bacteria were mostly recovered from both milk and tissue of the same quarter in significantly higher numbers from herd A cows compared with herd B. Molecular characterization of the isolates showed distinct PFGE profiles for isolates from each herd. Differences in virulence factors between herds A and B isolates were evidenced The genes for enterotoxin D, J and R were present in herd A, those for G, I, N, M, O and U were shown in herd B, whilst both components of the leukocidin lukD/E genes were only carried by herd A isolates. Furthermore, all herd A isolates showed β-haemolysin activity, which was absent in all but one isolate from herd B. Therefore our data indicate that Staph. aureus isolates showing differences in their ability to disseminate and colonize across quarters, also have significantly different virulence characteristics.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
S Agriculture > SF Animal culture
Uncontrolled Keywords: Staphylococcus aureus, bovine mastitis, molecular characteristics, mammary localization
Additional Information: Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0022-0299/ (accessed 25/02/2014).
Publisher: Cambridge University Press
ISSN: 0022-0299
Date of First Compliant Deposit: 30 March 2016
Last Modified: 04 Jun 2017 04:35
URI: http://orca-mwe.cf.ac.uk/id/eprint/41342

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