Residue choice defines efficiency and influence of bioorthogonal protein modification via genetically encoded strain promoted Click chemistry

Reddington, Samuel C., Tippmann, Eric Michael and Jones, Darran Dafydd ORCID: https://orcid.org/0000-0001-7709-3995 2012. Residue choice defines efficiency and influence of bioorthogonal protein modification via genetically encoded strain promoted Click chemistry. Chemical Communications 48 (67) , pp. 8419-8421. 10.1039/c2cc31887c

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Abstract

GFP and a FRET compatible dye were used to assess the influence of genetically encoded aryl azide positioning on Click chemistry-based protein conjugation. While modification efficiency of the sampled mutants using a strain promoted reaction varied by as much as [similar]10 fold, there was no simple correlation with accessibility of the aryl azide on GFP's surface. One labeled GFP mutant (Gln204AzPhe) exhibited high efficiency FRET ([similar]90%) and an unprecedented pseudo-Stokes shift of 126 nm.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Chemistry
Subjects:	Q Science > QD Chemistry
Publisher:	Royal Society of Chemistry
ISSN:	1359-7345
Last Modified:	08 Dec 2022 11:32
URI:	https://orca.cardiff.ac.uk/id/eprint/38925

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