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Production of nitric oxide in the synovial membrane of rheumatoid and osteoarthritis patients

McInnes, Iain B., Leung, Bernard P., Field, Max, Wei, Xiao-Qing, Huang, Fang-Ping, Sturrock, Roger D., Kinninmonth, Andrew, Weidner, Jeffrey, Mumford, Richard and Liew, Foo Y. 1996. Production of nitric oxide in the synovial membrane of rheumatoid and osteoarthritis patients. Journal Of Experimental Medicine 184 (4) , pp. 1519-1524.

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Abstract

We have demonstrated spontaneous nitric oxide (NO) production by primary synovial cultures from rheumatoid (RA) and osteoarthritis patients. Increased NO production followed addition of staphylococcal enterotoxin B. Immunochemical double staining with specific anti-human inducible NO synthase (iNOS) and nonspecific esterase (NSE), or anti-CD68 (markers for tissue macrophages) showed that although many lining layer cells in RA synovium expressed iNOS, most ('-'90%) were NSE- and CD68-, with only a minor population (~10%) which were iNOS +, CD68+/NSE § These data demonstrate the capacity for high output of NO by human synovial tissue and show that, although human macrophages can express high levels of iNOS, the majority of cells expressing iNOS are fibroblasts. We also report that synoviocytes, and macrophage cell lines, cultured with the NO donor, S-nitroso-acetyl penicillamine, produced high concentrations of tumor necrosis factor (TNF)-oe. These results suggest that NO may mediate pathology in P,A through the induction of TNF-ot production.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: R Medicine > RK Dentistry
Last Modified: 04 Jun 2017 04:16
URI: http://orca-mwe.cf.ac.uk/id/eprint/35643

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