Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Strain persistence of invasive Candida albicans in chronic hyperplastic candidosis that underwent malignant change

Williams, David Wynne, Bartie, K. L., Potts, Albert John Cornel, Wilson, Melanie, Fardy, Michael J. and Lewis, Michael Alexander Oxenham 2001. Strain persistence of invasive Candida albicans in chronic hyperplastic candidosis that underwent malignant change. Gerodontology 18 (2) , pp. 73-78. 10.1111/j.1741-2358.2001.00073.x

Full text not available from this repository.

Abstract

Objectives: The aim of this study was to assess persistence and tissue invasion of Candida albicans strains isolated from a 65 year-old patient with chronic hyperplastic candidosis (CHC), that subsequently developed into squamous cell carcinoma (SCC). Materials and Methods: C. albicans (n=7) were recovered from the oral cavity of the patient over seven years. Confirmation of CHC and SCC in this patient was achieved by histopathological examination of incisional biopsy tissue. DNA fingerprinting was performed on the seven isolates from the CHC patient together with a further eight isolates from patients with normal oral mucosa (n=2), chronic atrophic candidosis (n=1), SCC (n=1) and CHC (n=4). Genotyping involved the use of inter-repeat PCR using the eukaryotic repeat primer 1251. Characterisation of the tissue invasive abilities of the isolates was achieved by infecting a commercially available reconstituted human oral epithelium (RHE; SkinEthic, Nice, France). After 24 h. C. albicans tissue invasion was assessed by histopathological examination. Results: DNA fingerprinting demonstrated strain persistence of C. albicans in the CHC patient over a seven year period despite provision of systemic antifungal therapy. The strain of C. albicans isolated from this patient was categorised as a high invader within the RHE compared to other isolates. Conclusions: Candidal strain persistence was evident in a patient with CHC over seven years. This persistence may be due to incomplete eradication from the oral cavity following antifungal therapy or subsequent recolonisation from other body sites or separate exogenous sources. The demonstration of enhanced in vitro tissue invasion by this particular strain may, in part, explain the progression to carcinoma.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: R Medicine > RK Dentistry
Uncontrolled Keywords: Candida albicans; chronic hyperplastic candidosis; squamous cell carcinoma; DNA fingerprinting
Publisher: Wiley-Blackwell
ISSN: 0734-0664
Last Modified: 09 Jun 2019 22:20
URI: http://orca-mwe.cf.ac.uk/id/eprint/34559

Citation Data

Cited 16 times in Google Scholar. View in Google Scholar

Actions (repository staff only)

Edit Item Edit Item