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Detection of Candida in concentrated oral rinse cultures by real-time PCR

White, P. Lewis, Williams, David Wynne, Kuriyama, Tomoari, Samad, Shamim A., Lewis, Michael Alexander Oxenham and Barnes, Rosemary Ann 2004. Detection of Candida in concentrated oral rinse cultures by real-time PCR. Journal of clinical microbiology 42 (5) , pp. 2101-2107. 10.1128/JCM.42.5.2101-2107.2004

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Abstract

The incidence of oral candidosis has increased in recent years, largely as a result of the emergence of human immunodeficiency virus infection and the more widespread use of immunosuppressive chemotherapy. This development has been associated with a need for more reliable methods for the detection of Candida. The present study assessed the performance of a real-time PCR and two block-based PCRs for the detection of Candida in 193 concentrated oral rinse culture (CRC) specimens. A total of 102 CRC specimens were positive by culture for Candida; and 96, 90, and 75 of these were also positive by real-time, N18-specific, and internal transcribed spacer (ITS)-specific PCRs, respectively. The five false-negative results by the real-time PCR were all non-Candida albicans positive by culture. Of the 91 culture-negative CRC specimens, 20, 41, and 44 were positive by the real-time PCR and the N18- and ITS-specific PCRs, respectively. All three PCRs detected fungal DNA in 8 culture-negative CRC specimens, with a further 30 being positive by two of the three PCRs. A total of 32 CRC specimens were Candida free by all methods. In summary, a real-time PCR that provides a sensitive, specific, and rapid alternative technique for detection of Candida in the mouth is described.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Dentistry
Additional Information: Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0095-1137/ (accessed 25/02/2014)
Publisher: American Society for Microbiology
ISSN: 0095-1137
Date of First Compliant Deposit: 30 March 2016
Last Modified: 04 Jun 2017 01:31
URI: http://orca-mwe.cf.ac.uk/id/eprint/335

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