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Tear sample collection using cellulose acetate absorbent filters

Esmaeelpour Hajyar, Marieh, Cai, Jun, Watts, Patrick, Boulton, Michael Edwin and Murphy, Paul James 2008. Tear sample collection using cellulose acetate absorbent filters. Ophthalmic and Physiological Optics 28 (6) , pp. 577-583. 10.1111/j.1475-1313.2008.00603.x

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Abstract

PURPOSE: To assess the use of cellulose acetate filter rods as a technique for tear collection. METHOD: The cellulose acetate rod (CR) was compared with the 'standard' glass capillary tube (CT), in a series of experiments, to assess: sample collection by collected volume size; the effect of tear stimulation on total tear protein concentration and major tear protein concentrations; and technique invasiveness. RESULTS: No difference was found in concentrations for total protein, IgA (secretory immunoglobulin A), lactoferrin and lysozyme (p > 0.05) with no clinically significant increase in serum albumin to show serum leakage. Sample volume was higher for CR (p < 0.005) and sample volume increased for stimulated collection with CR (p = 0.001). Dilution effect of a stimulated sample size was reliably shown only with CR (r = -0.66, p = 0.011). Using bovine albumin standard with CR and CT, a smaller sample volume (p < 0.001) and a higher protein concentration (p < 0.001) were extracted with CR. CONCLUSION: The cellulose rod offers a suitable alternative to the glass CT. It is able to quickly absorb a sample, allowing use for a wide range of sample sizes, while being minimally invasive.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Uncontrolled Keywords: cellulose rod; glass capillary; tear collection; tear proteins
Publisher: Wiley-Blackwell
ISSN: 0275-5408
Last Modified: 09 Feb 2019 22:57
URI: http://orca-mwe.cf.ac.uk/id/eprint/33160

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