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Microneedle delivery of H5N1 influenza virus-like particles to the skin induces long-lasting B- and T-Cell responses in mice

Song, J. M., Kim, Y. C., Lipatov, A. S., Pearton, Marc, Davis, C. T., Yoo, D. G., Park, K. M., Chen, L. M., Quan, F. S., Birchall, James Caradoc, Donis, R. O., Prausnitz, M. R., Compans, R. W. and Kang, S. M. 2010. Microneedle delivery of H5N1 influenza virus-like particles to the skin induces long-lasting B- and T-Cell responses in mice. Clinical and Vaccine Immunology 17 (9) , pp. 1381-1389. 10.1128/CVI.00100-10

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Abstract

A simple method suitable for self-administration of vaccine would improve mass immunization, particularly during a pandemic outbreak. Influenza virus-like particles (VLPs) have been suggested as promising vaccine candidates against potentially pandemic influenza viruses, as they confer long-lasting immunity but are not infectious. We investigated the immunogenicity and protective efficacy of influenza H5 VLPs containing the hemagglutinin (HA) of A/Vietnam/1203/04 (H5N1) virus delivered into the skin of mice using metal microneedle patches and also studied the response of Langerhans cells in a human skin model. Prime-boost microneedle vaccinations with H5 VLPs elicited higher levels of virus-specific IgG1 and IgG2a antibodies, virus-specific antibody-secreting cells, and cytokine-producing cells up to 8 months after vaccination compared to the same antigen delivered intramuscularly. Both prime-boost microneedle and intramuscular vaccinations with H5 VLPs induced similar hemagglutination inhibition titers and conferred 100% protection against lethal challenge with the wild-type A/Vietnam/1203/04 virus 16 weeks after vaccination. Microneedle delivery of influenza VLPs to viable human skin using microneedles induced the movement of CD207(+) Langerhans cells toward the basement membrane. Microneedle vaccination in the skin with H5 VLPs represents a promising approach for a self-administered vaccine against viruses with pandemic potential.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Subjects: R Medicine > RM Therapeutics. Pharmacology
Additional Information: Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/1556-6811/ (accessed 24/02/2014)
Publisher: American Society for Microbiology
ISSN: 1556-6811
Date of First Compliant Deposit: 30 March 2016
Last Modified: 04 Jun 2017 04:05
URI: http://orca-mwe.cf.ac.uk/id/eprint/32374

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