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How to make DNA count: DNA-based diagnostic tools in veterinary parasitology

Hunt, P. W. and Lello, Joanne 2012. How to make DNA count: DNA-based diagnostic tools in veterinary parasitology. Veterinary Parasitology 186 (1-2) , pp. 101-108. 10.1016/j.vetpar.2011.11.055

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Abstract

Traditional methods for the diagnosis of parasitic helminth infections of livestock have a number of limitations, such as the inability to distinguish mixed-species infections, a heavy reliance on technical experience and also sub-sampling errors. Some of these limitations may be overcome through the development of rapid and accurate DNA-based tests. For example, DNA-based tests can specifically detect individual species in a mixed infection at either the larval or egg stages, in the absence of morphological differences among species. Even so, some diagnostic problems remain the same, irrespective of whether a DNA-based or traditional method is used. For example, sub-sampling errors from an aggregated distribution are likely to persist. It is proposed, however, that DNA-based diagnostic technologies offer an opportunity to expand diagnostic capabilities, and are discussed in the current review. The future introduction of DNA-based diagnostic technologies into routine diagnostic settings will also be discussed.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
S Agriculture > SF Animal culture
Uncontrolled Keywords: Diagnosis; DNA methods; Faecal egg count; Sheep; Goat; Cattle
Publisher: Elsevier
ISSN: 0304-4017
Last Modified: 04 Jun 2017 04:04
URI: http://orca-mwe.cf.ac.uk/id/eprint/32092

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