Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Modulation of CD59 expression by restrictive silencer factor-derived peptides in cancer immunotherapy for neuroblastoma

Donev, Rossen Mintchev, Gray, Lisa Clare, Baalasubramanian, Sivasankar, Hughes, Timothy Richard, Van Den Berg, Carmen Wilma and Morgan, Bryan Paul 2008. Modulation of CD59 expression by restrictive silencer factor-derived peptides in cancer immunotherapy for neuroblastoma. Cancer Research 68 (14) , pp. 5979-5987. 10.1158/0008-5472.CAN-07-6828

Full text not available from this repository.

Abstract

Tumor cells escape clearance by complement by abundantly expressing CD59 and other membrane complement regulators. Existing strategies for blocking/knocking down these regulators can contribute to tumor immunoclearance in vitro; however, there are numerous difficulties restricting their use in vivo. Here, we report a new strategy for suppression of CD59 expression in neuroblastoma using peptides that target regulators of CD59 expression. We identified the neural-restrictive silencer factor (REST) as a target for modulation of CD59 expression in neuroblastoma. We next designed plasmids that encoded peptides comprising different DNA-binding domains of REST and transfected them into neuroblastoma cell lines. These peptides suppressed CD59 expression, sensitizing neuroblastoma to complement-mediated killing triggered by anti-GD2 therapeutic monoclonal antibody. These CD59-modulating peptides might be effective therapeutic adjuvants to therapeutic monoclonal antibodies used for treatment of neuroblastoma and other cancer types sharing the same mechanism for regulation of CD59 expression.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Psychology
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords: CD59; complement killing; neuroblastoma; REST; therapeutic peptides
Publisher: American Association for Cancer Research
ISSN: 0008-5472
Last Modified: 09 Jun 2018 18:59
URI: http://orca-mwe.cf.ac.uk/id/eprint/29881

Citation Data

Cited 36 times in Google Scholar. View in Google Scholar

Cited 21 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item