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Membrane attack complex inhibitor CD59a protects against focal cerebral ischemia in mice

Harhausen, Denise, Khojasteh, Uldus, Stahel, Philip F, Morgan, Bryan Paul ORCID: https://orcid.org/0000-0003-4075-7676, Nietfeld, Wilfried, Dirnagl, Ulrich and Trendelenburg, George 2010. Membrane attack complex inhibitor CD59a protects against focal cerebral ischemia in mice. Journal of Neuroinflammation 7 , pp. 15-26. 10.1186/1742-2094-7-15

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Abstract

Background The complement system is a crucial mediator of inflammation and cell lysis after cerebral ischemia. However, there is little information about the exact contribution of the membrane attack complex (MAC) and its inhibitor-protein CD59. Methods Transient focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in young male and female CD59a knockout and wild-type mice. Two models of MCAO were applied: 60 min MCAO and 48 h reperfusion, as well as 30 min MCAO and 72 h reperfusion. CD59a knockout animals were compared to wild-type animals in terms of infarct size, edema, neurological deficit, and cell death. Results and Discussion CD59a-deficiency in male mice caused significantly increased infarct volumes and brain swelling when compared to wild-type mice at 72 h after 30 min-occlusion time, whereas no significant difference was observed after 1 h-MCAO. Moreover, CD59a-deficient mice had impaired neurological function when compared to wild-type mice after 30 min MCAO. Conclusion We conclude that CD59a protects against ischemic brain damage, but depending on the gender and the stroke model used.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Publisher: BioMed Central
ISSN: 1742-2094
Date of First Compliant Deposit: 30 March 2016
Last Modified: 13 May 2023 12:28
URI: https://orca.cardiff.ac.uk/id/eprint/28786

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