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A role for checkpoint kinase-dependent rad26 phosphorylation in transcription-coupled DNA repair in saccharomyces cerevisiae

Taschner, Michael, Harreman, Michelle, Teng, Yumin, Gill, Hefin, Anindya, Roy, Maslen, Sarah L., Skehel, J. Mark, Waters, Raymond and Svejstrup, Jesper Q. 2010. A role for checkpoint kinase-dependent rad26 phosphorylation in transcription-coupled DNA repair in saccharomyces cerevisiae. Molecular and Cellular Biology 30 (2) , pp. 436-446. 10.1128/MCB.00822-09

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Abstract

Upon DNA damage, eukaryotic cells activate a conserved signal transduction cascade known as the DNA damage checkpoint (DDC). We investigated the influence of DDC kinases on nucleotide excision repair (NER) in Saccharomyces cerevisiae and found that repair of both strands of an active gene is affected by Mec1 but not by the downstream checkpoint kinases, Rad53 and Chk1. Repair of the nontranscribed strand (by global genome repair) requires new protein synthesis, possibly reflecting the involvement of Mec1 in the activation of repair genes. In contrast, repair of the transcribed strand by transcription-coupled NER (TC-NER) occurs in the absence of new protein synthesis, and DNA damage results in Mec1-dependent but Rad53-, Chk1-, Tel1-, and Dun1-independent phosphorylation of the TC-NER factor Rad26, a member of the Swi/Snf group of ATP-dependent translocases and yeast homologue of Cockayne syndrome B. Mutation of the Rad26 phosphorylation site results in a decrease in the rate of TC-NER, pointing to direct activation of Rad26 by Mec1 kinase. These findings establish a direct role for Mec1 kinase in transcription-coupled repair, at least partly via phosphorylation of Rad26, the main transcription-repair coupling factor.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
Additional Information: Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0270-7306/ (accessed 25/02/2014)
Publisher: American Society for Microbiology
ISSN: 0270-7306
Date of First Compliant Deposit: 30 March 2016
Last Modified: 04 Jun 2017 03:49
URI: http://orca-mwe.cf.ac.uk/id/eprint/27558

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