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Critical stages of extracting DNA from aspergillus fumigatus in whole-blood specimens

White, P. Lewis, Perry, Michael D., Loeffler, Juergen, Melchers, Willem, Klingspor, Lena, Bretagne, Stephane, McCulloch, Elaine, Cuenca-Estrella, Manuel, Finnstrom, Niklas, Donnelly, J. Peter and Barnes, Rosemary Ann 2010. Critical stages of extracting DNA from aspergillus fumigatus in whole-blood specimens. Journal of Clinical Microbiology 48 (10) , pp. 3753-3755. 10.1128/JCM.01466-10

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Abstract

A standardized protocol for extracting DNA from Aspergillus fumigatus has been proposed by the European Aspergillus PCR Initiative (EAPCRI). Using meta-regression analysis, the EAPCRI showed certain stages of the process to be critical to providing a satisfactory analytical sensitivity. The study investigated each step of the EAPCRI protocol by elimination and monitored the influence on Aspergillus PCR performance.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > QR Microbiology
Additional Information: Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0095-1137/ (accessed 25/02/2014)
Publisher: American Society for Microbiology
ISSN: 0095-1137
Date of First Compliant Deposit: 30 March 2016
Last Modified: 04 Jun 2017 03:49
URI: http://orca-mwe.cf.ac.uk/id/eprint/27423

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