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Four-wave mixing of gold nanoparticles for three-dimensional cell microscopy

Langbein, Wolfgang Werner, Watson, Peter Duncan and Borri, Paola 2009. Four-wave mixing of gold nanoparticles for three-dimensional cell microscopy. Presented at: Lasers and Electro-Optics 2009 and the European Quantum Electronics Conference. CLEO Europe - EQEC 2009. European Conference on, Munich, Germany, 14-19 June 2009. Published in: Masia, Francesco, Langbein, Wolfgang Werner, Watson, Peter Duncan and Borri, Paola eds. 2009 Conference on Lasers & Electro-Optics Europe & 11th European Quantum Electronics Conference (CLEO EUROPE/EQEC 2009). Institute of Electrical and Electronics Engineers ( IEEE ), 10.1109/CLEOE-EQEC.2009.5191714

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Abstract

Optical microscopy is an indispensable tool for obtaining spatial and temporal resolution within living cells and tissues. In this paper, a novel multiphoton microscopy technique which exploits the third-order nonlinearity called four-wave mixing and applied to gold nanoparticles in resonance with their surface plasmon is developed. The coherent, transient and resonant nature of this signal allows the interferometric time-resolved detection of four-wave mixing specifically originating from GNPs, with particle diameters down to 5nm. This paper demonstrate high-contrast, background-free imaging of gold-labelled Golgi structures in HepG2 cells with a sub-diffraction-limit lateral (axial) resolution of 140nm (470nm) at excitation powers corresponding to less than 3K photothermal heating, compatible to live cell imaging. These results pave the way towards photostable non-toxic and highly sensitive in-vivo optical imaging with sub-micron three-dimensional resolution.

Item Type: Conference or Workshop Item (Paper)
Date Type: Publication
Status: Published
Schools: Biosciences
Physics and Astronomy
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
T Technology > T Technology (General)
T Technology > TA Engineering (General). Civil engineering (General)
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Publisher: Institute of Electrical and Electronics Engineers ( IEEE )
ISBN: 9781424440795
Last Modified: 07 Jun 2019 20:36
URI: http://orca-mwe.cf.ac.uk/id/eprint/27008

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