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Two-photon permeabilization and calcium measurements in cellular organelles

Gerasimenko, Oleg Vsevolodovich and Gerasimenko, Julia Vladimirovna 2010. Two-photon permeabilization and calcium measurements in cellular organelles. Live Cell Imaging: Methods and Protocols, Vol. 591. Methods in Molecular Biology, vol. 2. Springer, pp. 201-210. (10.1007/978-1-60761-404-3_12)

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Abstract

Inositol trisphosphate and cyclic ADP-ribose, main intracellular Ca2+ messengers, induce release from the intracellular Ca2+ stores via inositol trisphosphate and ryanodine receptors, respectively. Recently, studies using novel messenger nicotinic acid adenine dinucleotide phosphate (NAADP) releasing Ca2+ from calcium stores in organelles other than endoplasmic reticulum (ER) have been conducted. However, technical difficulties of Ca2+ measurements in relatively small Ca2+ stores prompted us to develop a new, more sensitive, and less damaging two-photon permeabilization technique. Applied to pancreatic acinar cells, this technique allowed us to show that all three messengers – IP3, cADPR, and NAADP – release Ca2+ from two intracellular stores: the endoplasmic reticulum and an acidic store in the granular region. This chapter describes a detailed procedure of using this technique with pancreatic acinar cells.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Publisher: Springer
ISBN: 978160761403-6
ISSN: 1064-3745
Last Modified: 04 Jun 2017 03:47
URI: http://orca-mwe.cf.ac.uk/id/eprint/26568

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