Langbein, Wolfgang Werner, Rocha-Mendoza, Israel, Masia, Francesco, Di Napoli, Claudia, Pope, Iestyn, Watson, Peter Duncan and Borri, Paola 2011. Differential CARS microscopy with linearly chirped femtosecond laser pulses. Presented at: Multiphoton Microscopy in the Biomedical Sciences XI, San Francisco, CA, USA, 23-25 January 2011. Published in: Periasamy, Ammasi, König, Karsten and So, Peter T. C. eds. Multiphoton Microscopy in the Biomedical Sciences XI. SPIE Proceedings SPIE, 79031I. 10.1117/12.873872 |
Official URL: http://proceedings.spiedigitallibrary.org/proceedi...
Abstract
We demonstrate frequency differential CARS (D-CARS) using femtosecond laser pulses linearly chirped by glass elements of high group-velocity dispersion. By replicating the Pump-Stokes pair into a pulse train at twice the laser repetition rate, and controlling the instantaneous frequency difference by glass dispersion, we adjust the Raman frequency probed by each pair in an intrinsically stable way. The resulting CARS intensities are detected simultaneously by a single photomultiplier as sum and difference using lock-in detection. We demonstrate imaging of living cells with strongly suppressed non-resonant background. We also show D-CARS using a single femtosecond laser source.
Item Type: | Conference or Workshop Item (Paper) |
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Date Type: | Publication |
Status: | Published |
Schools: | Biosciences Physics and Astronomy |
Subjects: | Q Science > Q Science (General) T Technology > T Technology (General) T Technology > TA Engineering (General). Civil engineering (General) T Technology > TK Electrical engineering. Electronics Nuclear engineering |
Publisher: | SPIE |
ISBN: | 9780819484406 |
ISSN: | 0277-786X |
Funders: | BBSRC, EPSRC |
Last Modified: | 07 Jul 2020 13:48 |
URI: | http://orca-mwe.cf.ac.uk/id/eprint/26010 |
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