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Mutant and wild type cell chemotaxis in 3D and 4D with ultrahigh- resolution optical coherence tomography.

Rey, Sara M., Harwood, Adrian John, Povazay, Boris, Hofer, Bernd, Unterhuber, Angelika and Drexler, Wolfgang 2010. Mutant and wild type cell chemotaxis in 3D and 4D with ultrahigh- resolution optical coherence tomography. Presented at: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV, San Francisco, CA, USA, 25-27 January 2010. Published in: Izatt, Joseph A., Fujimoto, James G. and Tuchin, Valery V. eds. Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV. Proceedings of SPIE , vol. 7554. Bellingham, WA: SPIE, pp. 505-509. 10.1117/12.843244

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Abstract

Conventionally, cell chemotaxis is studied on two-dimensional (2D) transparent surfaces due to limitations in optical and image data-collection techniques. However, substrates which more closely mimic the natural environment of cells are often opaque or three-dimensional (3D). The non-invasive label-free imaging technique of frequency domain optical coherence tomography (OCT) has high axial and transverse resolution of >4μm, comparatively high penetration depth and the ability to acquire volumes in a few seconds, therefore offering the potential to visualize moving cells in 3D (2D+time) and 4D (3D+time). Cell migration is demonstrated in 3D on opaque surfaces, and in 4D within an agarose gel. The speed and directionality of wild type (Ax2) cell movement is seen to be comparable on agar and nitrocellulose filter substrates. Differences can be clearly seen in the character of cell movement between Ax2, myosin knockout (mhcA-) and PLC null ~10μm Dictyostelium discoideum cells in 4D using ultrahigh resolution OCT. OCT is therefore shown to be a useful technique for the study of cell migration.

Item Type: Conference or Workshop Item (Paper)
Status: Published
Schools: Neuroscience and Mental Health Research Institute (NMHRI)
Biosciences
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
T Technology > T Technology (General)
T Technology > TA Engineering (General). Civil engineering (General)
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Uncontrolled Keywords: Opacity; Optical coherence tomography
Publisher: SPIE
ISBN: 9780819479501
Last Modified: 10 Oct 2017 14:11
URI: http://orca-mwe.cf.ac.uk/id/eprint/25975

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