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Visualization of 3D cell migration using high speed ultrahigh resolution optical coherence tomography

Rey, Sara Maria, Harwood, Adrian John, Povazay, Boris, Hofer, Bernd, Unterhuber, Angelika, Hermann, Boris and Drexler, Wolfgang 2009. Visualization of 3D cell migration using high speed ultrahigh resolution optical coherence tomography. Presented at: Optics in tissue engineering and regenerative medicine III, San Jose, CA, USA, 24 January 2009. Published in: Kirkpatrick, Sean J. and Wang, Ruikang eds. Optics in Tissue Engineering and Regenerative Medicine III, 24 January 2009, San Jose, CA, USA. Proceedings of SPIE , vol. 7179. Bellingham, WA: SPIE - International Society for Optical Engineering, pp. 505-509. 10.1117/12.809178

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Abstract

Using high speed ultrahigh resolution optical coherence tomography (OCT) at 800nm, non-invasive 3D cellular imaging has been accomplished. Cellular resolution imaging on and within these types of substrates is not possible with conventional microscopy techniques such as interference contrast microscopy, and requires the use of fluorescent staining. It is possible to achieve data acquisition rates of 20,000 samples per second with OCT which, in combination with its high axial and transverse resolution (>2-3μm), allows it to be used as a non-invasive technique to analyze cell migration in 3D with time. Comparatively high penetration depth also makes OCT a uniquely suited imaging technique for visualization of cells within a 3D construct. In this paper it is demonstrated that it is possible to resolve ~10μm Dictyostelium discoideum cells, a well established and useful model for investigation of cell motility and chemotaxis, in 3D and follow them in time lapse using an 800nm ultrahigh resolution high speed frequency domain based OCT microscope. Ultimately, these visualization techniques could enable monitoring of cell behavior in regenerative medicine, for example tracking of individual cells within a cell scaffold.

Item Type: Conference or Workshop Item (Paper)
Date Type: Publication
Status: Published
Schools: Neuroscience and Mental Health Research Institute (NMHRI)
Biosciences
Subjects: T Technology > T Technology (General)
T Technology > TA Engineering (General). Civil engineering (General)
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Uncontrolled Keywords: Optical coherence tomography; non invasive high resolution imaging; regenerative medicine; tissue engineering; cell migration; chemotaxis; cell morphogenesis
Publisher: SPIE - International Society for Optical Engineering
ISBN: 9780819474254
Last Modified: 10 Oct 2017 14:09
URI: http://orca-mwe.cf.ac.uk/id/eprint/25309

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