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Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection

Swann, Karl, Windsor, Shane, Campbell, Karen, Elgmati, Khalil, Nomikos, Michail, Zernicka-Goetz, Magdalena, Amso, Nazar Najib, Lai, Francis Anthony, Thomas, Adrian and Graham, Christopher 2012. Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection. Fertility and Sterility 97 (3) , pp. 742-747. 10.1016/j.fertnstert.2011.12.013

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Abstract

Objective: To evaluate the imaging of cytoplasmicmovements in human oocytes as a potential method to monitor the pattern of Ca2+oscillations during activation. Design: Test of a laboratory technique. Setting: University medical school research laboratory. Patient(s): Donated unfertilized human oocytes from intracytoplasmic sperm injection (ICSI) cycles. Intervention(s): Microinjection of oocytes with phospholipase C (PLC) zeta (ζ) cRNA and a Ca2+-sensitive fluorescent dye. Main Outcome Measure(s): Simultaneous detection of oocyte cytoplasmicmovements using particle image velocimetry (PIV) and of Ca2+oscillations using a Ca2+-sensitive fluorescent dye. Result(s): Microinjection of PLCζ cRNA into human oocytes that had failed to fertilize after ICSI resulted in the appearance of prolonged Ca2+oscillations. Each transient Ca2+ concentration change was accompanied by a small coordinated movement of the cytoplasm that could be detected using PIV analysis. Conclusion(s): The occurrence and frequency of cytoplasmic Ca2+oscillations, a critical parameter in activating human zygotes, can be monitored by PIV analysis of cytoplasmicmovements. This simple method provides a novel, noninvasive approach to determine in real time the occurrence and frequency of Ca2+oscillations in human zygotes.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > QL Zoology
R Medicine > RG Gynecology and obstetrics
Uncontrolled Keywords: oocyte, zygote, calcium, movement, phospholipase C zeta, cross correlation, particle image velocimetry
Publisher: Elsevier
ISSN: 0015-0282
Last Modified: 02 May 2019 11:37
URI: http://orca-mwe.cf.ac.uk/id/eprint/25263

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