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Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components

Sexton, Keith John, Balharry, Dominique Clare, Brennan, Paul, McLaren, James Edward, Brewis, Ian Andrew and Berube, Kelly Ann 2011. Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components. Biomarkers 16 (7) , pp. 567-576. 10.3109/1354750X.2011.608855

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Abstract

Historically, it has been challenging to go beyond epidemiology to investigate the pathogenic changes caused by tobacco smoking. The EpiAirway-100 (MatTek Corp., Ashland, MA) was employed to investigate the effects of cigarette smoke components. Exposure at the air-liquid-interface represented particle and vapour phase components of cigarette smoke. A proteomic study utilising iTRAQ labelling compared expression profiles. The correlative histopathology revealed focal regions of hyperplasia, hypertrophy, cytolysis and necrosis. We identified 466 proteins, 250 with a parameter of two or more peptides. Four of these proteins are potential markers of lung injury and three are related to mechanistic pathways of disease.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: biomarkers; lung; tobacco smoke; isobaric labeling; tandem mass spectrometry
Publisher: Informa
ISSN: 1354-750X
Last Modified: 07 Jul 2019 23:34
URI: http://orca-mwe.cf.ac.uk/id/eprint/24791

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