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Elucidation of the structure of a bioactive hydrophilic polysaccharide from Cordyceps sinensis by methylation analysis and NMR spectroscopy

Nie, Shao-Ping, Cui, Steve W., Phillips, Aled Owain, Xie, Ming-Yong, Phillips, Glyn O., Al-Assaf, Saphwan and Zhang, Xiao-Liang 2011. Elucidation of the structure of a bioactive hydrophilic polysaccharide from Cordyceps sinensis by methylation analysis and NMR spectroscopy. Carbohydrate Polymers 84 (3) , pp. 894-899. 10.1016/j.carbpol.2010.12.033

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Abstract

The structure of a bioactive hydrophilic polysaccharide fraction from Cordyceps sinensis (CBHP) was studied using methylation analysis and 2D NMR spectroscopy. Monosaccharide composition analysis revealed that it consists mainly of glucose (95.19%) with trace amounts of mannose (0.91%) and galactose (0.61%). The results of methylation analysis indicated that α-1,4 linked Glcp is the main linkage type (65.7%), followed by t-Glcp (20.7%), 1,2,3,6-Glcp (4.1%), 1,2,4,6-Glcp (3.0%), 1,3,6-Glcp (2.0%), 1,4,6-Glcp (1.6%), and 1,2-Manp (1.9%) and 1,3-Galp (1.0%). Based on 1D and 2D NMR analysis, a preliminary structure is proposed: The backbone is composed of Glcp joined by 1 → 4 linkages and 1 → 3 linkages; the branching points are located at O-2 or O-6 of Glcp with α- terminal-d-Glcp as side chain. The trace amounts of 1,2-Manp and 1, 3-Galp linkages are probably located randomly in the side chains. A schematic structure is proposed as following: Full-size image

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: Cordyceps sinensis; Hydrophilic polysaccharide; Structure; Methylation analysis; NMR
Publisher: Elsevier
ISSN: 0144-8617
Last Modified: 04 Jun 2017 03:38
URI: http://orca-mwe.cf.ac.uk/id/eprint/24158

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