Comparison of transcription-mediated amplification and growth-based methods for the quantitation of Enterococcus bacteria in environmental waters

Morris, Ceri, Blackwood, A. D., Kirs, M., Buttigieg, Neil D., Morgan, Rhian R., Hogan, J. J., Weeks, Ian and Noble, R. T. 2008. Comparison of transcription-mediated amplification and growth-based methods for the quantitation of Enterococcus bacteria in environmental waters. Applied and Environmental Microbiology 74 (10) , pp. 3319-3320. 10.1128/AEM.02623-07

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Abstract

An assay based on transcription-mediated amplification (TMA) technology was used to quantitate Enterococcus fecal indicator bacteria in environmental water samples. The results generated by this and two growth-based methods relative to the 104 most-probable-number or CFU-per-100-ml threshold show that the three methods are in good qualitative agreement when tested against a range of water samples taken from different locations. The results demonstrate sensitive and rapid detection (approximately 4 h from sample collection to result) and quantitation of Enterococcus bacteria compared to the results with the growth-based methods.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > Q Science (General)
Publisher:	ASM
ISSN:	0099-2240
Last Modified:	06 Jul 2020 14:30
URI:	http://orca-mwe.cf.ac.uk/id/eprint/23405

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