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Calcium imaging shows differential sensitivity to cooling and communication in luminous transgenic plants

Campbell, Anthony Keith, Trewavas, A. J. and Knight, M. R. 1996. Calcium imaging shows differential sensitivity to cooling and communication in luminous transgenic plants. Cell Calcium 19 (3) , pp. 211-218. 10.1016/S0143-4160(96)90022-6

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Abstract

Imaging of a recombinant bioluminescent Ca2+ indicator, aequorin, in an entire organism showed three novel features of Ca2+ signals in plants. First, cooling the plant from 25°C to 2°C demonstrated differentialsensitivities between organs, the roots firing a Ca2+ signal at some 8–10°C higher than the cotyledons. Secondly, prolonged cooling provoked Ca2+ oscillations, but only in the cotyledons. These oscillations occurred with a frequency of 100 s and damped down within 800 s. Thirdly, cooling the roots of mature plants triggered a Ca2+ signal in the leaves, as a result of organ-organ communication. However, warming and then recooling the roots did not generate a second Ca2+ signal in these leaves. This desensitisation was not due to down-regulation in the leaf since this was able to generate a Ca2+ signal of its own when cooled directly. Thus a combination of a recombinant bioluminescent indicator with photon counting imaging reveals startling new aspects of signalling in intact organs and whole organisms.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Pharmacy
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
R Medicine > RM Therapeutics. Pharmacology
Publisher: Elsevier
ISSN: 0143-4160
Last Modified: 05 Jun 2017 02:58
URI: http://orca-mwe.cf.ac.uk/id/eprint/22506

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