Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Evidence For De Novo Biosynthesis of Coelenterazine in the Bioluminescent Midwater Shrimp, Systellaspis Debilis C

Thomson, Catherine M., Herring, P. J. and Campbell, Anthony Keith 1995. Evidence For De Novo Biosynthesis of Coelenterazine in the Bioluminescent Midwater Shrimp, Systellaspis Debilis C. Journal of the Marine Biological Association of the United Kingdom 75 (1) , pp. 165-171. 10.1017/S0025315400015277

Full text not available from this repository.

Abstract

Coelenterazine chemiluminescence is now established as the most common chemistry responsible for bioluminescence in the sea, being found in seven phyla. However, the organisms which synthesize coelenterazine have yet to be identified. In order to deter-mine whether the luminous midwater shrimp Systellaspis debilis (A. Milne Edwards) (Arthropoda: Decapoda) is capable of luciferin biosynthesis, a developmental series of eggs was assayed for its luciferin, coelenterazine. The advantages of this system are that S. debilis eggs are autonomous and therefore have no external nutrient supply, the embryos can be ranked for developmental stage and the large egg size allows clutch numbers to be determined accurately. Recombinant apo-aequorin, which requires coelenterazine for luminescence, was used to quantify coelenterazine during the developmental sequence. An increase of almost two orders of magnitude was detected in coelenterazine content per egg between the first and final stage of development (mean values of 1 pmol and 71 pmol). This demonstrates de novo biosynthesis of coelenterazine for the first time.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Pharmacy
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
R Medicine > RM Therapeutics. Pharmacology
Publisher: Cambridge University Press
ISSN: 0025-3154
Last Modified: 05 Jun 2017 02:58
URI: http://orca-mwe.cf.ac.uk/id/eprint/22494

Citation Data

Cited 25 times in Google Scholar. View in Google Scholar

Cited 24 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item