Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Quantification of collagen organization in the peripheral human cornea at micron-scale resolution

Boote, Craig, Kamma-Lorger, Christina S., Hayes, Sally, Harris, Jonathan, Burghammer, Manfred, Hiller, Jennifer, Terrill, Nicholas J. and Meek, Keith Michael Andrew 2011. Quantification of collagen organization in the peripheral human cornea at micron-scale resolution. Biophysical Journal 101 (1) , pp. 33-42. 10.1016/j.bpj.2011.05.029

[img]
Preview
PDF
Download (1MB) | Preview

Abstract

The collagen microstructure of the peripheral cornea is important in stabilizing corneal curvature and refractive status. However, the manner in which the predominantly orthogonal collagen fibrils of the central cornea integrate with the circumferential limbal collagen is unknown. We used microfocus wide-angle x-ray scattering to quantify the relative proportion and orientation of collagen fibrils over the human corneolimbal interface at intervals of 50 μm. Orthogonal fibrils changed direction 1–1.5 mm before the limbus to integrate with the circumferential limbal fibrils. Outside the central 6 mm, additional preferentially aligned collagen was found to reinforce the cornea and limbus. The manner of integration and degree of reinforcement varied significantly depending on the direction along which the limbus was approached. We also employed small-angle x-ray scattering to measure the average collagen fibril diameter from central cornea to limbus at 0.5 mm intervals. Fibril diameter was constant across the central 6 mm. More peripherally, fibril diameter increased, indicative of a merging of corneal and scleral collagen. The point of increase varied with direction, consistent with a scheme in which the oblique corneal periphery is reinforced by chords of scleral collagen. The results have implications for the cornea's biomechanical response to ocular surgeries involving peripheral incision.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Publisher: Biophysical Society
ISSN: 0006-3495
Last Modified: 04 Jun 2017 03:32
URI: http://orca-mwe.cf.ac.uk/id/eprint/22427

Citation Data

Cited 19 times in Google Scholar. View in Google Scholar

Cited 33 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics