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An In Vitro Human Tissue Equivalent Model of Respiratory Epithelia for Toxicological Screening of Inhaled Nanoparticles

Berube, Kelly Ann, Hughes, Tracy G., Prytherch, Zoe Cariad, Sexton, Keith John, Koshy, Lata and Jones, Timothy Peter 2008. An In Vitro Human Tissue Equivalent Model of Respiratory Epithelia for Toxicological Screening of Inhaled Nanoparticles. Presented at: Nanotechnology: towards reducing animal testing, Institute of Nanotechnology, The Royal Society, London, UK, 28-29 May 2008.

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Abstract

Ethically-driven initiatives and the 2013 EU Cosmetic Directive banning repeat exposure tests in animals are making the need to develop alternative inhalation toxicology testing increasingly pressing. The work undertaken focused on toxicity testing of engineered nanoparticles used in aerosolized consumer products (e.g., pharmaceutical drugs, hair-sprays) and environmental NP (e.g. diesel exhaust particles, metal aerosols), with the aim of identifying key molecular biomarkers for the onset of damage in the lung. Normal human bronchial epithelial cells were tissue-engineered from medical waste (heart/lung transplant donors) to develop 3-dimensional cell cultures. Cultured cells formed tissue closely resembling epithelia of the human respiratory tract, with distinct cell types functioning as in vivo. NP can now be tested as though they have been inhaled and, given the endpoint data comes from human-derived, primary tissue instead of animal, there is no need to extrapolate results. Data is more representative of human lung responses/mechanisms and used with greater confidence in its accuracy. The model offers higher/quicker output in comparison to animal testing and provides a viable, low cost alternative to traditional toxicity testing methods.

Item Type: Conference or Workshop Item (Lecture)
Date Type: Publication
Status: Published
Schools: Biosciences
Earth and Ocean Sciences
Subjects: Q Science > Q Science (General)
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Additional Information: Post-Event Proceedings CD-ROM
Last Modified: 06 Nov 2018 21:37
URI: http://orca-mwe.cf.ac.uk/id/eprint/22092

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