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Activated leukocyte cell adhesion molecule (ALCAM) - biological impact on vascular endothelial cells [Abstract]

Minhas, Uzma, Sanders, Andrew James, Patel, Girish Khandubhai, Harding, Keith Gordon and Jiang, Wen Guo 2010. Activated leukocyte cell adhesion molecule (ALCAM) - biological impact on vascular endothelial cells [Abstract]. Wound Repair and Regeneration 18 (6) , A89. 10.1111/j.1524-475X.2010.00633.x

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Abstract

Our purpose was assess the impact of Activated Leucocyte Cell Adhesion Molecule (ALCAM) on Human Vascular Endothelial Cells (HECV), these cell are important in angiogenesis and hence wound healing. HECV line positive for ALCAM were used. Anti-ALCAM transgenes were constructed based on the secondary structure of the ALCAM mRAN. After transfection of HECV cells with the transgene and control vectors, the following cell sublines were created; a control (HECVpEF) endothelial cell line and a ALCAM-knockdown (HECVALCAM/KD) cell line. We then assessed the impact of ALCAM on cellular migration of the endothelial cells by way of electric wounding using Electrical Cell Impedence Sensing (ECIS) zy model. We successfully suppressed the expression of ALCAM in HECV cells by using the anti-ALCAM transgene. HECV rapidly migrated after wounding. It recovered at a rapid pace (0.57/min). Interestingly, after losing ALCAM transcript, the HECVALCAM/KD cells migrated at a substantially reduced rate. Finally, the levels of ALCAM in HECV cells did not affect the rate of cell growth, in vitro. Our results concur with those previously reported in cancer cells suggesting knockdown of ALCAM increases cellular migration and our results have shown this to also be true for endothelial cells. In terms of wound healing knockout of ALCAM in chronic wounds may provide a mechanism of increasing cellular migration and thus promoting wound healing.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
European Cancer Stem Cell Research Institute (ECSCRI)
Medicine
Subjects: R Medicine > R Medicine (General)
Additional Information: 20th Meeting of the European Tissue Repair Society Gent, Belgium September 15–17, 2010 Basic and Clinical Research: Building Blocks in the Puzzle of Tissue Repair
Publisher: Wiley-Blackwell
ISSN: 1067-1927
Last Modified: 04 Jun 2017 03:15
URI: http://orca-mwe.cf.ac.uk/id/eprint/18941

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