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The role of Bik1 (BCL-2 interaction killer) in wound healing [Abstract]

Rangaraj, Aravindan, Ye, Lin, Patel, Girish Khandubhai, Harding, Keith Gordon and Jiang, Wen Guo 2010. The role of Bik1 (BCL-2 interaction killer) in wound healing [Abstract]. Wound Repair and Regeneration 18 (6) , A92. 10.1111/j.1524-475X.2010.00633.x

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Abstract

To determine the role of Bik1 (BCL-2 interaction killer) a pro-apoptotic gene in Keratinocyte function and thus in wound healing Human keratinocyte cell line, HaCat wild type (WT), was transfected with anti-Bik1 ribozyme transgenes, constructed based on the secondary structure of Bik1 and specifically targeted human Bik1, in order to generate a HaCaT Bik1- knockdown subline. The successful knock down of the gene transcript was then verified with RT-PCR and Quantitative real time PCR (Q-PCR) alongside control HaCat WT and HaCat pEF cells. Cell functions, cell growth and cellular motility, were tested using a colorimetric growth assay and Electric cell-substrate impedance sensing (ECIS), respectively. RT-PCR and Q-PCR both verified the presence of Bik1 gene in the control cells and the knockdown of Bik1 transcript in cells transfected with the transgene. Growth assay demonstrated a significant(418%) increase in growth rate compared to both the control cells at day 5, po0.05. ECIS wounding assay confirmed that there was a significant (po0.05) increase in the cellular resistance over the 4 hr post-wounding period, reflecting a significant increase in cellular migration. Wound healing involves both proliferation and migration of specific cell populations that are essential for wound repair. Apoptosis allows for the eliminations without tissue damage or an inflammatory response. We observed the presence of pro-apoptotic Bik1 in keratinocyte cell line and that its deficiency can potentially increase the rate of cell growth and migration. This has a prospective benefit on the understanding of chronic wound phenotype and may prove therapeutic.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
European Cancer Stem Cell Research Institute (ECSCRI)
Medicine
Subjects: R Medicine > R Medicine (General)
Additional Information: 20th Meeting of the European Tissue Repair Society Gent, Belgium September 15–17, 2010 Basic and Clinical Research: Building Blocks in the Puzzle of Tissue Repair
Publisher: Wiley-Blackwell
ISSN: 1067-1927
Last Modified: 04 Jun 2017 03:15
URI: http://orca-mwe.cf.ac.uk/id/eprint/18938

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