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Evaluation of Bacillus subtilis strain IS53 for the production of Bacillus anthracis protective antigen

Baillie, Les, Johnson, M. and Manchee, R. J. 1994. Evaluation of Bacillus subtilis strain IS53 for the production of Bacillus anthracis protective antigen. Letters in Applied Microbiology 19 (4) , pp. 225-227. 10.1111/j.1472-765X.1994.tb00949.x

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Abstract

An asporogenous strain of Bacillus subtilis, IS53, transformed with plasmid pPA102, produces the protective antigen (PA) component of the tripartite toxin of B. anthracis. Addition of yeast extract was required to support growth and PA production in all the media examined. Protective antigen expression was down-regulated during exponential growth and extracellular proteases caused marked degradation of the mature protein.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Subjects: R Medicine > RM Therapeutics. Pharmacology
Publisher: Wiley-Blackwell
ISSN: 0266-8254
Last Modified: 04 Jun 2017 03:09
URI: http://orca-mwe.cf.ac.uk/id/eprint/17496

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