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Analysis of the genes for oestrogen and epidermal growth factor receptors in human breast cancer

Sharma, A. K., Grimshaw, D., Horgan, K., Neades, G. T., Gee, Julia Margaret Wendy, Douglas-Jones, Anthony Gordon, Mansel, R. E. and Nicholson, Robert Ian 1996. Analysis of the genes for oestrogen and epidermal growth factor receptors in human breast cancer. The Breast 5 (5) , pp. 344-350. 10.1016/S0960-9776(96)90002-8

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One of the consistent observations in the investigation of human breast cancer is the finding of an inverse relationship between oestrogen (ER) and epidermal growth factor (EGFR) receptors. The precise mechanisms responsible for this relationship are not fully understood. In order to examine whether genomic alterations make any contribution to this relationship we have examined the genes of these two receptors in 58 primary breast cancer patients using the techniques of Southern blotting and deoxyribonucleic acid (DNA) slot blotting and compared them to their respective proteins which were quantified using immunocytochemistry. Immunocytochemical analysis in 53 of these cases confirmed the existence of a strong inverse relationship between ER and EGFR (P < 0.001). DNA slot blot analysis revealed that the levels of both genes formed a normal distribution comparable to levels found in normal volunteers. Hence, no evidence of gene amplification for either ER or EGFR was observed. Southern blot analysis using EcoR1 restriction endonulcease digestion in 44 of these cases revealed no restriction fragment length polymorphisms (RFLPs) of the ER gene. RFLPs of the EGFR gene were observed but these have previously been demonstrated to be of no clinico-pathological relevance. From the results of this study it appears that amplification or alterations, using EcoR1 restriction endonuclease digestion, in either the ER or EGFR genes are unlikely to contribute to the inverse relationship in vivo. This contrasts with previous observations in tumour cell lines.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
R Medicine > RM Therapeutics. Pharmacology
Publisher: Elsevier
ISSN: 0960-9776
Last Modified: 04 Jun 2017 03:07

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