Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Accuracy of two root canal length measurement devices integrated into rotary endodontic motors when removing gutta-percha from root-filled teeth

Uzun, Orhan, Topuz, O., Tinaz, C., Nekoofar, Mohammad Hossein and Dummer, Paul Michael Howell 2008. Accuracy of two root canal length measurement devices integrated into rotary endodontic motors when removing gutta-percha from root-filled teeth. International Endodontic Journal 41 (9) , pp. 725-732. 10.1111/j.1365-2591.2008.01416.x

Full text not available from this repository.

Abstract

Using monomethoxy poly(ethylene glycol) (mPEG)-trypsin conjugates we recently showed that both PEG molecular weight (1100-5000 g/mol) and linker chemistry affect the rate of protein autolysis and thermal stability. These important factors are often overlooked but they can guide the early choice of optimal polymer/chemistry for synthesis of a lead polymer therapeutic suitable for later formulation development. As we are currently developing dextrin- and semi-telechelic poly[N-(2-hydroxypropyl)methacrylamide] (ST-HPMA)-protein conjugates as new therapeutics, the aim of this study was to examine the effect of polymer on activity, autolysis and its thermal stability using trypsin conjugates as a model and compare to the data obtained for mPEG conjugates. Trypsin conjugates were first synthesized using succinoylated dextrin (Mw ∼ 8000 g/mol, dextrin I; or ∼61,000 g/mol, dextrin II), and a ST-HPMA-COOH (Mw ∼ 10,100 g/mol). The conjugates had a trypsin content of ∼54, 17 and 3 wt% respectively with <5% free protein. When amidase activity (KM, Vmax and Kcat) was determined by using N-benzoyl-l-arginine p-nitroanilide (BAPNA) as substrate, trypsin KM values were not altered by conjugation, but the Vmax was ∼6-7-fold lower, and the substrate turnover rate (Kcat) decreased by ∼5-7-fold. The dextrin II-trypsin conjugate was more stable than the other conjugates and native trypsin at all temperatures between 30 and 70 °C, and also exhibited improved thermal stability in the autolysis assays at 40 °C. © 2009 Elsevier B.V. All rights reserved.

Item Type: Article
Status: Published
Schools: Dentistry
Medicine
Uncontrolled Keywords: automated root canal preparation; electronic apex locator; electronic root canal length measurement devices; retreatment; TCM Endo V®; Tri Auto ZX®
Publisher: Wiley & Sons
ISSN: 1365-2591
Last Modified: 04 Apr 2020 01:41
URI: http://orca-mwe.cf.ac.uk/id/eprint/16103

Citation Data

Cited 18 times in Google Scholar. View in Google Scholar

Cited 20 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item