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Translocation or just location? Pseudopodia affect fluorescent signals

Dewitt, Sharon, Darley, Richard Lawrence and Hallett, Maurice Bartlett 2009. Translocation or just location? Pseudopodia affect fluorescent signals. Journal of Cell Biology 184 (2) , pp. 197-203. 10.1083/jcb.200806047

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Abstract

The use of fluorescent probes is one of the most powerful techniques for gaining spatial and temporal knowledge of dynamic events within living cells. Localized increases in the signal from cytosolic fl uo rescent protein constructs, for example, are frequently used as evidence for translocation of proteins to specifi c sites within the cell. However, differences in optical and geometrical properties of cytoplasm can infl uence the recorded intensity of the probe signal. Pseudopodia are especially problematic because their cytoplasmic properties can cause abrupt increases in fl uorescent signal of both GFP and fl uorescein. Investigators should therefore be cautious when interpreting fl uorescence changes within a cell, as these can result from either translocation of the probe or changes in the optical properties of the milieu surrounding the probe.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Medicine
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Publisher: Rockefeller University Press
ISSN: 0021-9525
Last Modified: 04 Jun 2017 03:02
URI: http://orca-mwe.cf.ac.uk/id/eprint/15827

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