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Saccharomyces cerevisiae Rad16 mediates UV dependent histone H3 acetylation required for efficient global genome nucleotide excision repair

Teng, Yumin, Liu, Hairong, Gill, Hefin, Yu, Yachuan, Waters, Raymond and Reed, Simon Huw 2007. Saccharomyces cerevisiae Rad16 mediates UV dependent histone H3 acetylation required for efficient global genome nucleotide excision repair. [Technical Report]. EMBO Reports. Available at: http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retri...

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Abstract

In yeast, global genome nucleotide-excision repair (GG-NER) requires a protein complex containing Rad7 and Rad16. Rad16 is a member of the switch/sucrose nonfermentable superfamily, and it is presumed that chromatin remodelling is its primary function during repair. We show that RAD16 is required for ultraviolet-dependent hyperacetylation of histone H3 (Lys 9 and Lys 14) at the MFA2 promoter and throughout the genome. The yeast repressor complex Ssn6-Tup1 represses many genes including MFA2. TUP1 deletion results in constitutive hyperacetylation of histone H3, nucleosome disruption and derepression of gene transcription in Tup1-regulated genes. GG-NER in the MFA2 promoter proceeds more rapidly in tup1Delta alpha-cells compared with wild type, even when transcription is inhibited. We show that elevated histone H3 acetylation levels in the MFA2 promoter in tup1Delta alpha-cells result in Rad7- and Rad16-independent GG-NER, and that Rad16 mediates the ultraviolet-induced acetylation of histone H3, necessary for efficient GG-NER.

Item Type: Monograph (Technical Report)
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: Acetylation/radiation effects Adenosine Triphosphatases/metabolism* DNA Repair/radiation effects* DNA-Binding Proteins/metabolism Gene Deletion Gene Expression Regulation, Fungal/radiation effects Genes, Fungal Genome, Fungal/genetics* Genome, Fungal/radiation effects Histones/metabolism* Lipoproteins/genetics Lipoproteins/metabolism Nuclear Proteins/metabolism Pheromones Pyrimidine Dimers/radiation effects Repressor Proteins/metabolism Saccharomyces cerevisiae/enzymology* Saccharomyces cerevisiae/genetics* Saccharomyces cerevisiae/radiation effects Saccharomyces cerevisiae Proteins/genetics Saccharomyces cerevisiae Proteins/metabolism* Sequence Analysis, DNA Transcription, Genetic/radiation effects Ultraviolet Rays* Substances: DNA-Binding Proteins Histones Lipoproteins MFA2 protein, S cerevisiae Nuclear Proteins Pheromones Pyrimidine Dimers RAD7 protein, S cerevisiae Repressor Proteins Saccharomyces cerevisiae Proteins TUP1 protein, S cerevisiae Adenosine Triphosphatases RAD16 protein, S cerevisiae
Publisher: EMBO Reports
ISSN: 1469-221X
Last Modified: 31 Jan 2020 07:53
URI: http://orca-mwe.cf.ac.uk/id/eprint/146

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