Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Human skin is colonized by T cells that recognize CD1a independently of lipid

Cotton, Rachel N., Cheng, Tan-Yun, Wegrecki, Marcin, Le Nours, Jérôme, Orgill, Dennis P., Pomahac, Bohdan, Talbot, Simon G., Willis, Richard A., Altman, John D., de Jong, Annemieke, Ogg, Graham, Van Rhijn, Ildiko, Rossjohn, Jamie ORCID: https://orcid.org/0000-0002-2020-7522, Clark, Rachael A. and Moody, D. Branch 2021. Human skin is colonized by T cells that recognize CD1a independently of lipid. Journal of Clinical Investigation 131 (1) , e140706. 10.1172/JCI140706

Full text not available from this repository.

Abstract

CD1a-autoreactive T cells contribute to skin disease, but the identity of immunodominant self-lipid antigens and their mode of recognition are not yet solved. In most models, MHC and CD1 proteins serve as display platforms for smaller antigens. Here, we showed that CD1a tetramers without added antigen stained large T cell pools in every subject tested, accounting for approximately 1% of skin T cells. The mechanism of tetramer binding to T cells did not require any defined antigen. Binding occurred with approximately 100 lipid ligands carried by CD1a proteins, but could be tuned upward or downward with certain natural self-lipids. TCR recognition mapped to the outer A′ roof of CD1a at sites remote from the antigen exit portal, explaining how TCRs can bind CD1a rather than carried lipids. Thus, a major antigenic target of CD1a T cell autoreactivity in vivo is CD1a itself. Based on their high frequency and prevalence among donors, we conclude that CD1a-specific, lipid-independent T cells are a normal component of the human skin T cell repertoire. Bypassing the need to select antigens and effector molecules, CD1a tetramers represent a simple method to track such CD1a-specific T cells from tissues and in any clinical disease.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: American Society for Clinical Investigation
ISSN: 0021-9738
Date of Acceptance: 14 October 2020
Last Modified: 09 Nov 2022 10:05
URI: https://orca.cardiff.ac.uk/id/eprint/138207

Citation Data

Cited 15 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item