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Characterisation of progenitor cell populations from within the Oral Mucosa Lamina Propria

Roberts, James Thomas 2011. Characterisation of progenitor cell populations from within the Oral Mucosa Lamina Propria. MPhil Thesis, Cardiff University.
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Wound healing within the oral mucosa demonstrates “foetal-like” responses with minimal scarring. This preferential healing has been attributed to the phenotypic characteristics of Oral Mucosa Fibroblasts (OMF), which display enhanced replicative potential. Adult stem cell populations discovered throughout the body including the oral cavity led to the discovery of a novel stem cell population within the lamina propria. This investigation aims to characterise this stem cell population in comparison with populations isolated from the lamina propria by different means, in an attempt to describe the preferential properties of a progenitor cell population within the tissue. Differential adhesion to fibronectin facilitated the isolation of clonally derived stem cell populations (clone x), a cell population representing the whole lamina propria, analogous to OMF (LO), and cells which do not adhere to fibronectin within the context of the differential adhesion assay (NA). Population doublings indicated that all cell populations were capable of exceeding the growth potential of BMSCs, however populations demonstrated distinct growth characteristics during the early phase of growth. All cell populations demonstrated an increase in cell size over time in culture, associated with replicative senescence. Active telomerase was detected in all cell populations; however this was not sufficient to prevent cellular senescence in any cell population, with telomere degradation apparent in many cell populations. xiii Differentiation was achieved in all cell populations, with temporal differences in gene expression observed during adipogenesis. The success of the theoretically progenitor depleted NA population to successfully undergo differentiation suggests that the current isolation method, clonal expansion after fibronectin adherence, may not be the most practical means by which to enrich for a stem cell population from the Lamina Propria of the Oral Mucosa.

Item Type: Thesis (MPhil)
Status: Unpublished
Schools: Dentistry
Subjects: R Medicine > RK Dentistry
Funders: Richard Whipp studentship
Date of First Compliant Deposit: 30 March 2016
Last Modified: 19 Mar 2016 22:22

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