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Cell jamming, stratification and p63 expression in cultivated human corneal epithelial cell sheets

Baba, Koichi, Sasaki, Kei, Morita, Mio, Tanaka, Tomoyo, Teranishi, Yosuke, Ogasawara, Takahiro, Oie, Yoshinori, Kusumi, Izumi, Inoie, Masukazu, Hata, Ken-ichiro, Quantock, Andrew J., Kino-oka, Masahiro and Nishida, Kohji 2020. Cell jamming, stratification and p63 expression in cultivated human corneal epithelial cell sheets. Scientific Reports 10 (1) , 9282. 10.1038/s41598-020-64394-6

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Abstract

Corneal limbal epithelial stem cell transplantation using cultivated human corneal epithelial cell sheets has been used successfully to treat limbal stem cell deficiencies. Here we report an investigation into the quality of cultivated human corneal epithelial cell sheets using time-lapse imaging of the cell culture process every 20 minutes over 14 days to ascertain the level of cell jamming, a phenomenon in which cells become smaller, more rounded and less actively expansive. In parallel, we also assessed the expression of p63, an important corneal epithelial stem cell marker. The occurrence of cell jamming was variable and transient, but was invariably associated with a thickening and stratification of the cell sheet. p63 was present in all expanding cell sheets in the first 9 days of culture, but it’s presence did not always correlate with stratification of the cell sheet. Nor did p63 expression necessarily persist in stratified cell sheets. An assessment of cell jamming, therefore, can shed significant light on the quality and regenerative potential of cultivated human corneal epithelial cell sheets.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Optometry and Vision Sciences
Publisher: Nature Publishing Group
ISSN: 2045-2322
Date of First Compliant Deposit: 28 September 2020
Date of Acceptance: 24 February 2020
Last Modified: 28 Sep 2020 10:00
URI: http://orca-mwe.cf.ac.uk/id/eprint/135137

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