Hedegaard, Anne, Monzón-Sandoval, Jimena, Newey, Sarah E., Whiteley, Emma S., Webber, Caleb and Akerman, Colin J.
2020.
Pro-maturational effects of human iPSC-derived cortical astrocytes upon iPSC-derived cortical neurons.
Stem Cell Reports
15
, pp. 1-14.
10.1016/j.stemcr.2020.05.003
![]() |
![]() |
PDF
- Published Version
Available under License Creative Commons Attribution. Download (5MB) |
Abstract
Astrocytes influence neuronal maturation and function by providing trophic support, regulating the extracellular environment, andmodulating signaling at synapses. The emergence of induced pluripotent stem cell (iPSC) technology offers a human system with whichto validate and re-evaluate insights from animal studies. Here, we set out to examine interactions between human astrocytes and neuronsderived from a common cortical progenitor pool, thereby recapitulating aspects ofin vivocortical development. We show that the corticaliPSC-derived astrocytesexhibit many of the molecular and functional hallmarks of astrocytes. Furthermore, optogenetic and electrophys-iological co-culture experiments reveal that the iPSC-astrocytes can actively modulate ongoing synaptic transmission and exertpro-maturational effects upon developing networks of iPSC-derived cortical neurons. Finally, transcriptomic analyses implicate syn-apse-associated extracellular signaling in the astrocytes’ pro-maturational effects upon the iPSC-derived neurons. This work helps laythe foundation for future investigations into astrocyte-to-neuron interactions in human health and disease.
Item Type: | Article |
---|---|
Date Type: | Publication |
Status: | Published |
Schools: | Medicine |
Publisher: | Elsevier |
ISSN: | 2213-6711 |
Date of First Compliant Deposit: | 23 June 2020 |
Date of Acceptance: | 4 May 2020 |
Last Modified: | 31 Jul 2020 16:14 |
URI: | http://orca-mwe.cf.ac.uk/id/eprint/132690 |
Actions (repository staff only)
![]() |
Edit Item |